Systemic administration of antigen/peptide for peripheral T cell tolerance has lengthy been investigated as a possible method to remedy of autoimmune illnesses.
The a number of antimyelin T cell reactivities more likely to be related to a number of sclerosis (MS) impose main difficulties in devising such an immune-specific therapeutic method to the illness, as a result of focusing on T cells particular for a single autoantigen/epitope is unlikely to be sufficiently efficient.
Right here, we current a pilot examine on the potential for concomitantly inhibiting a number of doubtlessly pathogenic antimyelin T cell reactivities by tolerogenic administration of a man-made “multiantigen/multiepitope” protein. An artificial gene was constructed to encode chosen disease-relevant epitopes of myelin primary protein (MBP), proteolipid protein (PLP), and myelin oligodendrocyte glycoprotein (MOG).
The protein product, hmTAP (artificial human multitarget autoantigen protein), was adequately processed for antigenic presentation of the related integral epitopes, in vitro and in vivo.
Systemic administration of hmTAP not solely suppressed and handled experimental autoimmune encephalomyelitis (EAE) initiated by autoreactivity to a PLP epitope, but additionally abrogated advanced EAE transferred by multispecific line T cells reactive towards encephalitogenic epitopes of MBP, PLP, and MOG.
These knowledge point out that multiantigen/multiepitope-directed remedy of advanced autoimmune illnesses is efficient and will be mediated by the protein product of a particularly designed artificial gene.
[The correlation between expression of oncogene protein products p53, p21, p185 and cell differentiation and prognosis in rhabdomyosarcoma].
OBJECTIVETo examine the correlation between expression of oncogene proteinproducts p53, P21, p185 and histological kind, cell differentiation and prognosis in rhabdomyosarcoma (RMS).METHODS41 RMS circumstances which had follow-up materials had been chosen for this examine.
Expression of proteinproducts of oncogene p53, p21 and p185 had been synchronously detected and in contrast by immunohistochemical ABC methodology.RESULTSThe optimistic charges for p53, p21 ras and P185 c-erbB-2 had been 72%, 68% and 60% respectively. Constructive expression didn’t relate to age, intercourse or RMS histological kind, however associated to the diploma of RMS differentiation.
The optimistic charge of p53 advert p21 ras in nicely differentiated circumstances had been 42.9% and 28.6% whereas that of the poorly differentiated group was 85% and 80% respectively (P < 0.05). The psoitive charge of p53 within the RMS group with metastasis was 86.6%, considerably greater than that of the non-metastasized group, which was 66.7% (P < 0.05).
There was a major distinction between these with one 12 months survival, whose p52 optimistic charge was 86.7% and people who survived for greater than three years, whose p53 optimistic charge was 47.1% (P < 0.05).CONCLUSIONSThe outcomes counsel that the irregular expressions of p53 and p21 had been associated to tumor differentiation and the diploma of malignancy. p53 positivity might point out a poor prognosis.