VAPB (vesicle-associated membrane protein-associated protein B) is a tail-anchored protein that’s current at a number of contact websites of the endoplasmic reticulum (ER). We now present by immunoelectron microscopy that VAPB additionally localizes to the inside nuclear membrane (INM). Utilizing a modified APEX2 (enhanced ascorbate peroxidase 2)-approach with rapamycin-dependent concentrating on of the peroxidase to a protein of curiosity, we looked for proteins which might be in shut proximity to VAPB, notably on the INM.
Together with steady isotope labeling with amino acids in cell tradition (SILAC), we confirmed many well-known interplay companions on the stage of the ER with a transparent distinction between particular and non-specific hits. Moreover, we recognized emerin, TMEM43 and ELYS as potential interplay companions of VAPB on the INM and the nuclear pore complicated, respectively.
Identification of recent transmembrane proteins concentrated on the nuclear envelope utilizing organellar proteomics of mesenchymal cells.
The double membrane nuclear envelope (NE), which is contiguous with the ER, incorporates nuclear pore complexes (NPCs) – the channels for nucleocytoplasmic transport, and the nuclear lamina (NL) – a scaffold for NE and chromatin group. Since quite a few human illnesses linked to NE proteins happen in mesenchyme-derived cells, we used proteomics to characterize NE and different subcellular fractions remoted from mesenchymal stem cells and from adipocytes and myocytes.
Primarily based on spectral abundance, we calculated enrichment scores for proteins within the NE fractions. We demonstrated by quantitative immunofluorescence microscopy that 5 little-characterized proteins with excessive enrichment scores are considerably concentrated on the NE, with Itprip uncovered on the outer nuclear membrane, Smpd4 enriched on the NPC, and Mfsd10, Tmx4, and Arl6ip6 probably residing within the inside nuclear membrane. These proteins present new focal factors for finding out the capabilities of the NE. Furthermore, our datasets present a useful resource for evaluating further potential NE proteins.
Quantitative Proteomics Identifies DNA Restore as a Novel Organic Operate for Hepatocyte Nuclear Issue 4α in Colorectal Most cancers Cells.
Hepatocyte nuclear issue 4α (HNF4α) is a transcription issue that acts as a grasp regulator of genes for a number of endoderm-derived tissues, together with the gut, wherein it performs a central function throughout growth and tumorigenesis. To raised outline the mechanisms by which HNF4α can affect these processes, we recognized proteins interacting with HNF4α utilizing steady isotope labelling with amino acids in cell tradition (SILAC)-based quantitative proteomics with both immunoprecipitation of inexperienced fluorescent protein (GFP) or with proximity-dependent purification by the biotin ligase BirA (BioID), each fused to HNF4α.
Surprisingly, these analyses recognized a big enrichment of proteins characterised with a job in DNA restore, a thus far unidentified organic characteristic of this transcription issue. A number of of those proteins together with PARP1, RAD50, and DNA-PKcs had been confirmed to work together with HNF4α in colorectal most cancers cell traces. Following DNA harm, HNF4α was capable of improve cell viability in colorectal most cancers cells. General, these observations establish a possible function for this transcription issue in the course of the DNA harm response.
Purposeful Proteomics of Nuclear Proteins in Tetrahymena thermophila: A Assessment.
Identification and characterization of protein complexes and interactomes has been important to the understanding of basic nuclear processes together with transcription, replication, recombination, and upkeep of genome stability. Regardless of vital progress in elucidation of nuclear proteomes and interactomes of organisms equivalent to yeast and mammalian programs, progress in different fashions has lagged.
Protists, together with the alveolate ciliate protozoa with Tetrahymena thermophila as some of the studied members of this group, have a singular nuclear biology, and nuclear dimorphism, with structurally and functionally distinct nuclei in a typical cytoplasm. These options have been necessary in offering necessary insights about quite a few basic nuclear processes.
Right here, we assessment the proteomic approaches that had been traditionally used in addition to these at the moment employed to reap the benefits of the distinctive biology of the ciliates, specializing in Tetrahymena, to deal with necessary questions and higher perceive nuclear processes together with chromatin biology of eukaryotes.
Using proteomic strategy to establish nuclear translocation associated serine kinase phosphorylation web site of GNMT as downstream effector for benzo[a]pyrene.
Glycine N-methyltransferase (GNMT) protein is very expressed in sure tissues, equivalent to liver, pancreas, and prostate. GNMT serves a number of roles which embody a methyl group switch enzyme and a liver tumor suppressor. Benzo(a)pyrene (BaP), a member of the family of polycyclic fragrant hydrocarbon (PAH), is a recognized environmental carcinogen present in coal tar, tobacco smoke, barbecued meals and incomplete combustion of auto gas. BaP recruits cytochrome P450 to rework itself into benzo(a)pyrene-7,8-diol-9,10-epoxide (B(a)PDE), which covalently interacts with DNA inflicting tumorigenesis. BaP could be detoxified by means of GNMT and induces GNMT translocation into the mobile nucleus.
GNMT translocation is accompanied by phosphorylation, however the function of phosphorylation in GNMT stays to be explored. Utilizing liquid chromatography coupled with tandem mass spectrometry, this examine recognized serine 9 of GNMT because the phosphorylation web site upon BaP therapy. When serine 9 was mutated and misplaced the aptitude to be phosphorylated, the prevalence of BaP-induced GNMT nuclear translocation was dramatically decreased.
Rat S. Muscles 2 Months Total Protein |
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| RT-102-M2 | Zyagen | 1mg | EUR 177 |
Rat S. Muscles 3 Months Total Protein |
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| RT-102-M3 | Zyagen | 1mg | EUR 177 |
Rat S. Muscles 6 Months Total Protein |
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| RT-102-M6 | Zyagen | 1mg | EUR 177 |
Human Skeletal Muscles Lysate, Total Protein |
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| Skeletal-Muscles-012H | Creative BioMart | 100ug | EUR 158.4 |
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Description: Stored at -80 centigrade. |
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Mouse S. Muscles, match set of RNA, DNA, Protein |
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| MS-102-RDP | Zyagen | 20µg/20µg/100µg | EUR 422 |
Dog Skeletal Muscles Total Protein |
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| DT-102 | Zyagen | 1mg | EUR 176 |
Cat Skeletal Muscles Total Protein |
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| FT-102 | Zyagen | 1mg | EUR 176 |
Rat Skeletal Muscles Total Protein |
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| RT-102 | Zyagen | 1mg | EUR 153 |
Pig Skeletal Muscles Total Protein |
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| PT-102 | Zyagen | 1mg | EUR 153 |
Sheep Skeletal Muscles Total Protein |
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| ST-102 | Zyagen | 1mg | EUR 153 |
Bovine Skeletal Muscles Total Protein |
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| BT-102 | Zyagen | 1mg | EUR 153 |
Equine Skeletal Muscles Total protein |
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| ET-102 | Zyagen | 1mg | EUR 176 |
Rabbit Skeletal Muscles Total Protein |
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| TT-102 | Zyagen | 1mg | EUR 153 |
Hamster Skeletal Muscles Total Protein |
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| AT-102 | Zyagen | 1mg | EUR 153 |
Chicken Skeletal Muscles Total Protein |
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| CT-102 | Zyagen | 1mg | EUR 153 |
MiniPig Skeletal Muscles Total Protein |
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| NT-102 | Zyagen | 1mg | EUR 176 |
Human Troponin C, Slow Skeletal and Cardiac Muscles (TNNC1) Protein |
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| abx680515-10g | Abbexa | 10 µg | EUR 225 |
Human Troponin C, Slow Skeletal and Cardiac Muscles (TNNC1) Protein |
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| abx680515-50g | Abbexa | 50 µg | EUR 325 |
Rat WS Skeletal Muscles Total Protein |
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| RT-102-WS | Zyagen | 1mg | EUR 153 |
Human Skeletal Muscles Total RNA |
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| HR-102 | Zyagen | 0.025mg | EUR 172 |
Mouse CD1 Skeletal Muscles Total Protein |
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| MT-102 | Zyagen | 1mg | EUR 153 |
Mouse BLC Skeletal Muscles Total Protein |
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| MT-102-BLC | Zyagen | 1mg | EUR 180 |
Mouse C57 Skeletal Muscles Total Protein |
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| MT-102-C57 | Zyagen | 1mg | EUR 180 |
Human Skeletal Muscles Genomic DNA |
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| HG-102 | Zyagen | 0.05mg | EUR 210 |
Guinea Pig Skeletal Muscles Total Protein |
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| GT-102 | Zyagen | 1mg | EUR 153 |
CASQ2 (untagged)-Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein |
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| SC119365 | Origene Technologies GmbH | 10 µg | Ask for price |
Monkey Skeletal Muscles Total Protein, Rhesus |
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| UT-102 | Zyagen | 1mg | EUR 176 |
Human Skeletal Muscles Frozen Sections |
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| HF-102 | Zyagen | 10slides | EUR 240 |
Human Skeletal Muscles Paraffin Sections |
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| HP-102 | Zyagen | 10 slides | EUR 228 |
Recombinant Human Troponin C, slow skeletal and cardiac muscles (TNNC1) Protein |
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| MBS286212-01mg | MyBiosource | 0.1mg | EUR 440 |
Recombinant Human Troponin C, slow skeletal and cardiac muscles (TNNC1) Protein |
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| MBS286212-05mg | MyBiosource | 0.5mg | EUR 1200 |
Recombinant Human Troponin C, slow skeletal and cardiac muscles (TNNC1) Protein |
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| MBS286212-1mg | MyBiosource | 1mg | EUR 1665 |
Recombinant Human Troponin C, slow skeletal and cardiac muscles (TNNC1) Protein |
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| MBS286212-5x1mg | MyBiosource | 5x1mg | EUR 7480 |
CASQ2 (GFP-tagged) - Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein |
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| RG204695 | Origene Technologies GmbH | 10 µg | Ask for price |
Monkey Skeletal Muscles Total Protein, Cynomolgus |
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| KT-102 | Zyagen | 1mg | EUR 176 |
Human Skeletal Muscles cDNA-Oligo-dT |
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| HD-102 | Zyagen | 30 reactions | EUR 280 |
CASQ2 (Myc-DDK-tagged)-Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein |
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| RC204695 | Origene Technologies GmbH | 10 µg | Ask for price |
Human Skeletal Muscles cDNA-Random Primer |
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| HD-102-HR | Zyagen | 30 reactions | EUR 280 |
Recombinant Human Musculoskeletal, Embryonic Nuclear Protein 1 |
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| MBS141096-0002mg | MyBiosource | 0.002mg | EUR 240 |
Recombinant Human Musculoskeletal, Embryonic Nuclear Protein 1 |
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| MBS141096-001mg | MyBiosource | 0.01mg | EUR 310 |
Recombinant Human Musculoskeletal, Embryonic Nuclear Protein 1 |
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| MBS141096-01mg | MyBiosource | 0.1mg | EUR 1360 |
Recombinant Human Musculoskeletal, Embryonic Nuclear Protein 1 |
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| MBS141096-5x01mg | MyBiosource | 5x0.1mg | EUR 5800 |
CASQ1 (untagged)-Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein |
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| SC324617 | Origene Technologies GmbH | 10 µg | Ask for price |
CASQ1 (untagged)-Human calsequestrin 1 (fast-twitch skeletal muscle) (CASQ1) nuclear gene encoding mitochondrial protein |
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| SC327824 | Origene Technologies GmbH | 10 µg | Ask for price |
CASQ1 (untagged)-Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein |
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| SC119363 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, mGFP tagged |
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| RC204695L2 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, mGFP tagged |
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| RC204695L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Total Protein Extraction Kit for Muscles (50 tests) |
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| P5A06 | 101Bio | - | Ask for price |
Human Musculoskeletal Embryonic Nuclear Protein 1 (MUSTN1) ELISA Kit |
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| abx533779-96tests | Abbexa | 96 tests | EUR 687.5 |
Human Musculoskeletal embryonic nuclear protein 1(MUSTN1) Elisa Kit |
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| EK713075 | AFG Bioscience LLC | 96 Wells | EUR 0.32 |
Human Musculoskeletal embryonic nuclear protein 1,MUSTN1 ELISA KIT |
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| E5706Hu-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | EUR 4122 |
Human Musculoskeletal embryonic nuclear protein 1,MUSTN1 ELISA KIT |
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| E5706Hu-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | EUR 300 |
Human Musculoskeletal embryonic nuclear protein 1,MUSTN1 ELISA KIT |
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| E5706Hu-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | EUR 2061 |
Human Musculoskeletal embryonic nuclear protein 1,MUSTN1 ELISA KIT |
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| E5706Hu-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human Musculoskeletal embryonic nuclear protein 1,MUSTN1 ELISA KIT |
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| JOT-EK5706Hu | Jotbody | 96 wells | Ask for price |
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Description: Human |
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Human Musculoskeletal embryonic nuclear protein 1, MUSTN1 ELISA Kit |
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| MBS1606600-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human Musculoskeletal embryonic nuclear protein 1, MUSTN1 ELISA Kit |
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| MBS1606600-48StripWells | MyBiosource | 48-Strip-Wells | EUR 285 |
Human Musculoskeletal embryonic nuclear protein 1, MUSTN1 ELISA Kit |
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| MBS1606600-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human Musculoskeletal embryonic nuclear protein 1, MUSTN1 ELISA Kit |
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| MBS1606600-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
Musculoskeletal, Embryonic Nuclear Protein 1 Human Recombinant |
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| rAP-4206 | Angio Proteomie | Inquiry | Ask for price |
CASQ1 (GFP-tagged) - Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein |
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| RG204696 | Origene Technologies GmbH | 10 µg | Ask for price |
Human MUSTN1 / Musculoskeletal embryonic nuclear protein 1 ELISA Kit |
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| E6725h | EIAab | 96T | EUR 736 |
Recombinant Human Musculoskeletal embryonic nuclear protein 1 (MUSTN1) |
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| MBS1459395-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1005 |
Recombinant Human Musculoskeletal embryonic nuclear protein 1 (MUSTN1) |
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| MBS1459395-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 575 |
Recombinant Human Musculoskeletal embryonic nuclear protein 1 (MUSTN1) |
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| MBS1459395-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 760 |
Recombinant Human Musculoskeletal embryonic nuclear protein 1 (MUSTN1) |
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| MBS1459395-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 685 |
Recombinant Human Musculoskeletal embryonic nuclear protein 1 (MUSTN1) |
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| MBS1459395-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 885 |
MUSTN1 (untagged)-Human musculoskeletal, embryonic nuclear protein 1 (MUSTN1) |
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| SC308241 | Origene Technologies GmbH | 10 µg | Ask for price |
Human Musculoskeletal embryonic nuclear Protein 1, MUSTN1 GENLISA ELISA |
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| KBH5706 | Krishgen | 1 x 96 wells | EUR 286 |
Lenti ORF clone of Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
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| RC204695L1 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
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| RC204695L3 | Origene Technologies GmbH | 10 µg | Ask for price |
COX7A1 (untagged)-Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein |
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| SC118989 | Origene Technologies GmbH | 10 µg | Ask for price |
CASQ1 (Myc-DDK-tagged)-Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein |
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| RC204696 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF particles, CASQ2 (Myc-DDK tagged) - Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, 200ul, |
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| RC204695L1V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CASQ2 (mGFP-tagged) - Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, 200ul, >1 |
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| RC204695L2V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CASQ2 (Myc-DDK tagged) - Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, 200ul, |
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| RC204695L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CASQ2 (mGFP-tagged) - Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, 200ul, >1 |
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| RC204695L4V | Origene Technologies GmbH | 200 µl | Ask for price |
MUSTN1 (GFP-tagged) - Human musculoskeletal, embryonic nuclear protein 1 (MUSTN1) |
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| RG214142 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein, mGFP tagged |
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| RC204696L2 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein, mGFP tagged |
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| RC204696L4 | Origene Technologies GmbH | 10 µg | Ask for price |
MinuteTM Total Protein Extraction Kit for Muscles (50 tests) |
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| SA-06-MS | Inventbiotech | each | EUR 399 |
CPT1B (untagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 1 |
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| SC109096 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (untagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 7 |
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| SC326176 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (untagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 5 |
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| SC326207 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (untagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 6 |
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| SC325323 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (untagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 8 |
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| SC325324 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (untagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 3 |
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| SC309483 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (untagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 2 |
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| SC314660 | Origene Technologies GmbH | 10 µg | Ask for price |
COX7A1 (GFP-tagged) - Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein |
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| RG201154 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein, mGFP tag |
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| RC201154L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF particles, CPT1B (mGFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, |
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| RC217297L2V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (mGFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, |
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| RC217297L4V | Origene Technologies GmbH | 200 µl | Ask for price |
CPT1B (GFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 3 |
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| RG222144 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (GFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 2 |
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| RG214823 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (GFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 5 |
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| RG227244 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (GFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 6 |
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| RG227262 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (GFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 7 |
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| RG227280 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (GFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 8 |
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| RG227313 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (GFP-tagged) - Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 1 |
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| RG217297 | Origene Technologies GmbH | 10 µg | Ask for price |
COX7A1 (Myc-DDK-tagged)-Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein |
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| RC201154 | Origene Technologies GmbH | 10 µg | Ask for price |
MUSTN1 (Myc-DDK-tagged)-Human musculoskeletal, embryonic nuclear protein 1 (MUSTN1) |
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| RC214142 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
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| RC204696L1 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
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| RC204696L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF particles, CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, t |
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| RC214823L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, t |
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| RC227244L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, t |
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| RC227262L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, t |
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| RC227280L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, t |
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| RC227313L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, t |
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| RC222144L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Rat Skeletal Muscles Developmental Total Protein Panel, 6 stages |
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| RT-102-006D | Zyagen | 6x0.1mg | EUR 998 |
Lenti ORF clone of Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein, Myc-DDK- |
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| RC201154L3 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant |
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| RC214823 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant |
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| RC227244 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant |
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| RC227262 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant |
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| RC227280 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant |
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| RC227313 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant |
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| RC222144 | Origene Technologies GmbH | 10 µg | Ask for price |
CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant |
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| RC217297 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, |
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| RC214823L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, tra |
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| RC214823L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, |
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| RC227244L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, tra |
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| RC227244L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, |
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| RC227262L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, tra |
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| RC227262L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, |
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| RC227280L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, tra |
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| RC227280L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, |
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| RC227313L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, tra |
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| RC227313L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, |
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| RC222144L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti-ORF clone of CPT1B (mGFP-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, tra |
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| RC222144L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 1, M |
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| RC217297L1 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 1, m |
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| RC217297L2 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 1, M |
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| RC217297L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 1, m |
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| RC217297L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF particles, CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein |
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| RC214823L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein |
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| RC227244L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein |
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| RC227262L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein |
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| RC227280L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein |
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| RC227313L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein |
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| RC222144L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Dog Skeletal Muscles RNA |
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| DR-102 | Zyagen | 0.05mg | EUR 195 |
Dog Skeletal Muscles cDNA |
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| DD-102 | Zyagen | 30 Reactions | EUR 280 |
Cat Skeletal Muscles cDNA |
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| FD-102 | Zyagen | 30 Reactions | EUR 280 |
Pig Skeletal Muscles cDNA |
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| PD-102 | Zyagen | 30 reactions | EUR 243 |
Additionally, this mutant from of GNMT misplaced the power of phosphorylation and elevated cytochrome P450 1A1 (Cyp1a) expression upon BaP therapy. As well as, protein kinase C (PKC) and c-Jun NH2-terminal kinase (JNK) could also be required for such phosphorylation. Additional characterization of phosphorylated GNMT for its hyperlink to BaP could carry new insights into chemical detoxing.