(Phospho)proteomic Profiling of Microsatellite Unstable CRC Cells Reveals Alterations in Nuclear Signaling and Cholesterol Metabolism Caused by Frameshift Mutation of NMD Regulator UPF3A

(Phospho)proteomic Profiling of Microsatellite Unstable CRC Cells Reveals Alterations in Nuclear Signaling and Cholesterol Metabolism Caused by Frameshift Mutation of NMD Regulator UPF3A

DNA mismatch repair-deficient colorectal cancers (CRCs) accumulate quite a few frameshift mutations at repetitive sequences acknowledged as microsatellite instability (MSI). When coding mononucleotide repeats (cMNRs) are affected, tumors accumulate frameshift mutations and untimely termination codons (PTC) doubtlessly resulting in truncated proteins.
Nonsense-mediated RNA decay (NMD) can degrade PTC-containing transcripts and defend from such defective proteins. Because it additionally regulates regular transcripts and mobile physiology, we examined whether or not NMD genes themselves are targets of MSI frameshift mutations.
A excessive frequency of cMNR frameshift mutations within the UPF3A gene was present in MSI CRC cell traces (67.7%), MSI colorectal adenomas (55%) and carcinomas (63%). In regular colonic crypts, UPF3A expression was restricted to single chromogranin A-positive cells. SILAC-based proteomic evaluation of KM12 CRC cells revealed UPF3A-dependent down-regulation of a number of enzymes concerned in ldl cholesterol biosynthesis.
Moreover, reconstituted UPF3A expression brought about alterations of 85 phosphosites in 52 phosphoproteins. Most of them (38/52, 73%) reside in nuclear phosphoproteins concerned in regulation of gene expression and RNA splicing. Since UPF3A mutations can modulate the (phospho)proteomic signature and expression of enzymes concerned in ldl cholesterol metabolism in CRC cells, UPF3A might affect different processes than NMD and lack of UPF3A expression would possibly present a progress benefit to MSI CRC cells.

Proximity labeling proteomics reveals important regulators for interior nuclear membrane protein degradation in crops

The interior nuclear membrane (INM) selectively accumulates proteins which can be important for nuclear features; nevertheless, overaccumulation of INM proteins leads to a spread of uncommon genetic problems. To date, little is understood about how faulty, mislocalized, or abnormally amassed membrane proteins are actively faraway from the INM, particularly in crops and animals.
Right here, through evaluation of a proximity-labeling proteomic profile of INM-associated proteins in Arabidopsis, we establish important elements for an INM protein degradation pathway. We present that this pathway depends on the CDC48 complicated for INM protein extraction and 26S proteasome for subsequent protein degradation.
Furthermore, we present that CDC48 on the INM could also be regulated by a subgroup of PUX proteins, which decide the substrate specificity or have an effect on the ATPase exercise of CDC48. These PUX proteins particularly affiliate with the nucleoskeleton beneath the INM and bodily work together with CDC48 proteins to negatively regulate INM protein degradation in crops.

TMT-based Quantitative Proteomics Evaluation Reveals the Attenuated Replication Mechanism of Newcastle Illness Virus Attributable to Nuclear Localization Sign Mutation in Viral Matrix Protein

Nuclear localization of cytoplasmic RNA virus proteins mediated by intrinsic nuclear localization sign (NLS) performs important roles in profitable virus replication. We beforehand reported that NLS mutation within the matrix (M) protein clearly attenuates the replication and pathogenicity of Newcastle illness virus (NDV), however the attenuated replication mechanism stays unclear.
On this research, we confirmed that M/NLS mutation not solely disrupted M’s nucleocytoplasmic trafficking attribute but in addition impaired viral RNA synthesis and transcription. Utilizing TMT-based quantitative proteomics evaluation of BSR-T7/5 cells contaminated with the parental NDV rSS1GFP and the mutant NDV rSS1GFP-M/NLSm harboring M/NLS mutation, we discovered that rSS1GFP an infection stimulated a lot larger portions and extra expression modifications of differentially expressed proteins concerned in host cell transcription, ribosomal construction, posttranslational modification, and intracellular trafficking than rSS1GFP-M/NLSm an infection.
Additional in-depth evaluation revealed that the dominant nuclear accumulation of M protein inhibited host cell transcription, RNA processing and modification, protein synthesis, posttranscriptional modification and transport; and this type of inhibition may very well be weakened when most of M protein was confined exterior the nucleus.
(Phospho)proteomic Profiling of Microsatellite Unstable CRC Cells Reveals Alterations in Nuclear Signaling and Cholesterol Metabolism Caused by Frameshift Mutation of NMD Regulator UPF3A
Extra importantly, we discovered that the operate of M protein within the cytoplasm effected the inhibition of TIFA expression in a dose-dependent method, and promoted NDV replication by down-regulating TIFA/TRAF6/NF-κB-mediated manufacturing of cytokines. It was the primary report in regards to the involvement of M protein in NDV immune evasion.
Taken collectively, our findings show that NDV replication is intently associated to the nucleocytoplasmic trafficking of M protein, which accelerates our understanding of the molecular features of NDV M protein.

Hepatic Transporter Alterations by Nuclear Receptor Agonist T0901317 in Sandwich-cultured Human Hepatocytes: Proteomic Evaluation and PBPK Modeling to Consider Drug-Drug Interplay Danger.

In vitro approaches for predicting drug-drug interactions (DDIs) attributable to alterations in transporter protein regulation usually are not properly established. Nonetheless, studies of transporter regulation through nuclear receptor (NR) modulation by medicine are growing. This research examined alterations in transporter protein ranges in sandwich-cultured human hepatocytes (SCHH; n = three donors) measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic evaluation after therapy with T0901317, the primary described artificial liver X receptor (LXR) agonist.
T0901317 therapy (10 μM, 48 h) decreased the degrees of natural cation transporter (OCT) 1 (0.22-, 0.43- and 0.71-fold of management) and natural anion transporter (OAT) 2 (0.38-, 0.38- and 0.53-fold of management), and elevated multidrug resistance protein (MDR) 1 (1.37-, 1.48 and 1.59-fold of management).
The induction of NR downstream gene expression help the speculation that T0901317 off-target results on farnesoid X receptor (FXR) and pregnane X receptor (PXR) activation are liable for the sudden modifications in OCT1, OAT2 and MDR1. Uptake of the OCT1 substrate metformin in SCHH was decreased by T0901317 therapy.
Results of decreased OCT1 ranges on metformin had been simulated utilizing a physiologically-based pharmacokinetic (PBPK) mannequin. Simulations confirmed a transparent lower in metformin hepatic publicity leading to a decreased pharmacodynamic impact.
This DDI wouldn’t be predicted by the modest modifications in simulated metformin plasma concentrations. Altogether, the present research demonstrated that an strategy combining SCHH, proteomic evaluation, and PBPK modeling is helpful for revealing tissue concentration-based DDIs on account of sudden regulation of hepatic transporters by NR modulators.
SIGNIFICANCE STATEMENT: This research utilized an strategy combining sandwich-cultured human hepatocytes, proteomic evaluation, and physiologically-based pharmacokinetic modeling to guage alterations in pharmacokinetics (PK) and pharmacodynamics (PD) on account of transporter regulation by nuclear receptor modulators.

Alexa Fluor® 594 anti-mouse CD45

E16FMJ045-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD68

E16FMJ068-100U 100 μg
EUR 1365
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD8a

E16FHJ008a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD14

E16FHJ0142-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD19

E16FHJ019-100U 100 μg
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD11c

E16FMJ011c-100U 100 μg
EUR 758.33
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD106

E16FMJ106-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD11a

E16FHJ011a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD11b

E16FHJ011b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Mouse Anti-Human IgA - Alexa Fluor 594

DL87387A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

DL87387A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

DL87390A-100ul 100 ul
EUR 155
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

DL87390A-500ul 500 ul
EUR 350
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

E38A3634 100μL
EUR 125

Mouse Anti-Human IgM - Alexa Fluor 594

E38A3638 100μL
EUR 125

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-1ml 1 ml
EUR 480
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-500ul 500 ul
EUR 290
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

DL87379A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

DL87379A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

E38A3625 100μL
EUR 125

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

DL87358A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

DL87358A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

E38A3604 100ug/100ul
EUR 125
Description: Available in various conjugation types.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

DL87357A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

DL87357A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

DL87389A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

DL87389A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-mouse CD206 (MMR)

E16FMJ206-100U 100 μg
EUR 1191.67
Description: Available in various conjugation types.

Goat Anti-Human IgG Fab - Alexa Fluor 594

E38A3603 100μL
EUR 125

Mouse Anti-Human IgG Fc - Alexa Fluor 594

E38A3636 100μL
EUR 125

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

DL87376A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

DL87376A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

DL87388A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

DL87388A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-human/mouse CD44

E16FMJ044-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

E38A3622 100μL
EUR 125

Mouse Anti-Human IgG Fab - Alexa Fluor 594

E38A3635 100μL
EUR 125

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-human/mouse CD11b

E16FMJ011b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF 500 ug
EUR 334
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF 500 ug
EUR 465
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87364A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87364A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3610 100μL
EUR 125

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF 500 ug
EUR 333
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87393A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87393A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3641 100μL
EUR 125

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

DL87356A-100ul 100 ul
EUR 140
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

DL87356A-500ul 500 ul
EUR 330
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

DL87362A-100ul 100 ul
EUR 140
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

DL87362A-500ul 500 ul
EUR 330
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

DL87367A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

DL87367A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

DL87369A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

DL87369A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87382A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87382A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

DL87383A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

DL87383A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Duck IgY (H&L) - Alexa Fluor 594

DL87386A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Duck IgY (H&L) - Alexa Fluor 594

DL87386A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

E38A3602 100μL
EUR 125

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

E38A3608 100μL
EUR 125

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

E38A3613 100μL
EUR 125

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

E38A3615 100μL
EUR 125

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3628 100μL
EUR 125

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

E38A3629 100μL
EUR 125

Mouse Anti-Duck IgY (H&L) - Alexa Fluor 594

E38A3633 100μL
EUR 125

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

YLF0109-100ul 100 ul
EUR 100
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

YLF0109-1ml 1 ml
EUR 490
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

YLF0109-500ul 500 ul
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

YLF0110-100ul 100 ul
EUR 100
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

YLF0110-1ml 1 ml
EUR 490
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

YLF0110-500ul 500 ul
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

YLF0345-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

YLF0345-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

YLF0345-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

YLF0347-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

YLF0347-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

YLF0347-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0358-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0358-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0358-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
The significance of this strategy from a mechanistic and clinically-relevant perspective is that it could actually reveal drug-drug interactions (DDIs) on account of sudden regulation of hepatic transporters, and allow prediction of altered PK and PD modifications, particularly for tissue concentration-based DDIs.
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