Handled with gentle pulse or beneath sure diurnal situations, photoreceptors can translocate into nucleus adopted by conformation change. Many vital elements of sunshine signaling pathways additionally majorly perform in nucleus. Therefore, it’s helpful to ascertain a mixed methodology to uncover and evaluate the nuclear proteomic panorama among the many mutants of sunshine signaling elements.
Right here we describe an optimized methodology to isolate nucleus with seedlings rising beneath gentle/darkish cycles for additional characterizing the nuclear proteome with label-free quantitation by liquid chromatography mass spectrometry (LC-MS).
Proteomics of Herpes Simplex Virus Sort 1 Nuclear Capsids
Herpes simplex virus replicates within the nucleus, the place new capsids are assembled. It produces procapsids devoid of nucleic acid however containing the preVP22a scaffold protein. These thermo-unstable particles then mature into A-, B- or C-nuclear icosahedral capsids, relying on their potential to shed the proteolytically processed scaffold and incorporation of the viral genome.
To review how these viral capsids differ, we carried out proteomics research of extremely enriched HSV-1 A-, B- and C-nuclear capsids, relying partially on a novel and highly effective circulate virometry method to purify C-capsids. We discovered that the viral particles contained the anticipated capsid elements and recognized a number of tegument proteins within the C-capsid fraction (pU<sub>L</sub>21, pU<sub>L</sub>36, pU<sub>L</sub>46, pU<sub>L</sub>48, pU<sub>L</sub>49, pU<sub>L</sub>50, pU<sub>L</sub>51 and pU<sub>S</sub>10).
Furthermore, quite a few ribosomal, hnRNPs and different host proteins, absent from the uninfected controls, have been detected on the capsids with a few of them seemingly particular to C-capsids (glycogen synthase, 4 completely different keratin-related proteins, fibronectin 1 and PCBP1). A subsequent proteomics evaluation was carried out to rule out the presence of protein complexes which will share related density because the viral capsids however don’t in any other case work together with them.
Utilizing pUL25 or VP5 mutant viruses incapable of assembling C-nuclear or all nuclear capsids, respectively, we confirmed the majority of our preliminary findings. Naturally, it is going to subsequent be vital to handle the purposeful relevance of those proteins.<b>IMPORTANCE</b> A lot is thought in regards to the biology of herpesviruses. This consists of their distinctive potential to traverse the 2 nuclear envelopes by sequential budding and fusion steps. For HSV-1, this means the pU<sub>L</sub>31/pU<sub>L</sub>34 and pU<sub>L</sub>17/pU<sub>L</sub>25 complexes which will favor C-capsid egress.
Nonetheless, this choice course of just isn’t clear, nor are all of the variations that distinguish A-, B- and C-capsids. The current examine probes what proteins compose these capsids, together with host proteins. This could open up new analysis avenues to make clear the biology of this most fascinating household of viruses. It additionally reiterates using circulate virometry as an revolutionary instrument to purify viral particles.
A Novel Proteomic Methodology Reveals NLS Tagging of T-DM1 Contravenes Classical Nuclear Transport in a Mannequin of HER2-Constructive Breast Most cancers
The following breakthrough for protein therapeutics is efficient intracellular supply and accumulation inside goal cells. Nuclear localization sign (NLS)-tagged therapeutics have been hindered by the shortage of environment friendly nuclear localization because of endosome entrapment.
Though improvement of methods for tagging therapeutics with applied sciences able to elevated membrane penetration has resulted in proportional elevated efficiency, nonspecific membrane penetration limits goal specificity and, therefore, widespread medical success. There’s a long-standing concept that nuclear localization of NLS-tagged brokers happens completely by way of classical nuclear transport.
Within the current examine, we modified the antibody-drug conjugate trastuzumab-emtansine (T-DM1) with a classical NLS linked to cholic acid (cell accumulator [Accum]) that permits modified antibodies to flee endosome entrapment and enhance nuclear localization effectivity with out abrogating receptor focusing on. In parallel, we developed a proteomics-based methodology to judge nuclear transport.
Accum-modified T-DM1 considerably enhanced cytotoxic efficacy within the human epidermal development issue receptor 2 (HER2)-positive SKBR3 breast most cancers system. We found that efficacy was depending on the nonclassical importin-7.
Our analysis reveals that when a number of classical NLS tagging happens, cationic cost build-up versus sequence dominates and turns into a substrate for importin-7. This examine leads to an efficient goal cell-specific NLS therapeutic and a normal method to information future NLS-based improvement initiatives.
Affinity proteomic dissection of the human nuclear cap-binding complicated interactome
A 5′,7-methylguanosine cap is a quintessential function of RNA polymerase II-transcribed RNAs, and a textbook side of co-transcriptional RNA processing. The cap is sure by the cap-binding complicated (CBC), canonically consisting of nuclear cap-binding proteins 1 and a couple of (NCBP1/2). Curiosity within the CBC has not too long ago renewed because of its participation in RNA-fate selections by way of interactions with RNA productive components in addition to with adapters of the degradative RNA exosome.
A novel cap-binding protein, NCBP3, was not too long ago proposed to type an alternate CBC along with NCBP1, and to work together with the canonical CBC together with the protein SRRT. The theme of post-transcriptional RNA destiny, and the way it pertains to co-transcriptional ribonucleoprotein meeting, is plentiful with difficult, ambiguous, and certain incomplete fashions.
Rat Embryo PrimaCell1: Normal Embryonic Chorion Cells Growth Medium |
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| 9-25031 | CHI Scientific | 5 x 100 ml | Ask for price |
Rat Thymocyte Nuclear Protein 1 (THYN1) Protein |
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| 20-abx650182 | Abbexa |
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Rat Embryo Tissue Preparation Buffer 1: Normal Embryonic Chorion Cells |
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| 9-80238 | CHI Scientific | 1 x 100 ml | Ask for price |
Chick Embryo Extract, Lyophilized |
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| C1163-010 | GenDepot | 10ml | EUR 481.2 |
Mouse 3T3 (NIH), embryo lysate |
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| MCL-1282 | Alpha Diagnostics | 100ug | EUR 255.6 |
Rat Embryo PrimaCell2: Normal Fetal Dermal Fibroblasts |
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| 2-82532 | CHI Scientific | 1 Kit | Ask for price |
CLC/Ipomoea Medium, Embryo Production |
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| CP006-010 | GenDepot | 10X1L | EUR 118.8 |
CLC/Ipomoea Medium, Embryo Production |
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| CP006-500 | GenDepot | 50L | EUR 151.2 |
ELISA kit for Rat Nuclear protein MDM1 (MDM1) |
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| KTE100643-48T | Abbkine | 48T | EUR 398.4 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear protein MDM1 (MDM1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Rat Nuclear protein MDM1 (MDM1) |
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| KTE100643-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear protein MDM1 (MDM1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Rat Nuclear protein MDM1 (MDM1) |
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| KTE100643-96T | Abbkine | 96T | EUR 646.8 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear protein MDM1 (MDM1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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Rat Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes |
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| 2-82533 | CHI Scientific | 1 Kit | Ask for price |
Mouse Embryo PrimaCell1: Normal Embryonic Chorion Cells |
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| 2-82030 | CHI Scientific | 1 Kit | Ask for price |
Human Embryo PrimaCell1: Normal Embryonic Chorion Cells |
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| 2-96050 | CHI Scientific | 1 Kit | Ask for price |
Rat Sp100 Nuclear Antigen (Sp100) Protein |
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| 20-abx168513 | Abbexa |
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Rat Nuclear Factor I/X (NFIX) Protein |
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| 20-abx650671 | Abbexa |
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CMV protein (nuclear) |
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| 30-AC98 | Fitzgerald | 1 mg | EUR 548.4 |
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Description: Purified native CMV protein (nuclear) |
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Rat Thymocyte nuclear protein 1(THYN1) ELISA kit |
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| E02T0692-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat Thymocyte nuclear protein 1(THYN1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Thymocyte nuclear protein 1(THYN1) ELISA kit |
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| E02T0692-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Thymocyte nuclear protein 1(THYN1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Thymocyte nuclear protein 1(THYN1) ELISA kit |
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| E02T0692-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Thymocyte nuclear protein 1(THYN1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Nuclear protein 1 Antibody |
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| abx018122-100ug | Abbexa | 100 ug | EUR 410.4 |
Rat Nuclear Matrix Protein 66 ELISA kit |
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| E02N0031-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear Matrix Protein 66 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear Matrix Protein 66 ELISA kit |
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| E02N0031-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear Matrix Protein 66 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear Matrix Protein 66 ELISA kit |
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| E02N0031-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear Matrix Protein 66 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear matrix protein 22 ELISA kit |
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| E02N0590-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear matrix protein 22 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear matrix protein 22 ELISA kit |
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| E02N0590-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear matrix protein 22 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear matrix protein 22 ELISA kit |
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| E02N0590-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear matrix protein 22 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Thymocyte Nuclear Protein 1 Protein |
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| 20-abx261394 | Abbexa |
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Rat Nuclear Factor Kappa B (NFkB) Protein |
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| 20-abx168498 | Abbexa |
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Rat Embryo PrimaCell4: Normal Trophoblast Cells Growth Medium |
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| 9-25034 | CHI Scientific | 5 x 100 ml | Ask for price |
Nuclear Protein Extraction Kit |
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| BSP009 | Bio Basic | 50Preps | EUR 106.98 |
Total RNA - Rat Normal Tissue: Embryo (RtRAN: 17-19 days) |
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| R1434000-50 | Biochain | 50 ug | EUR 209 |
Mouse 3T3 (Balb), murine embryo lysate |
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| MCL-1212 | Alpha Diagnostics | 100 ug | EUR 255.6 |
Rat Nuclear Respiratory Factor 1 (NRF1) Protein |
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| 20-abx654593 | Abbexa |
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Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat) |
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| 4-PAC791Ra01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1) |
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Rat Nuclear RNA Export Factor 1 (NXF1) Protein |
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| 20-abx168840 | Abbexa |
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Nuclear Protein 1 (NUPR1) Antibody |
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| abx430003-200ul | Abbexa | 200 ul | EUR 343.2 |
Nuclear Protein 1 (NUPR1) Antibody |
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| 20-abx302286 | Abbexa |
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Rat Embryo Tissue Preparation Buffer 4: Normal Trophoblast Cells |
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| 9-80241 | CHI Scientific | 1 x 100 ml | Ask for price |
Mdm1 Nuclear Protein (MDM1) Antibody |
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| abx235075-100ug | Abbexa | 100 ug | EUR 610.8 |
Mdm1 Nuclear Protein (MDM1) Antibody |
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| 20-abx216769 | Abbexa |
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Mdm1 Nuclear Protein (MDM1) Antibody |
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| 20-abx113633 | Abbexa |
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Mdm1 Nuclear Protein (MDM1) Antibody |
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| abx026744-400ul | Abbexa | 400 ul | EUR 627.6 |
Mdm1 Nuclear Protein (MDM1) Antibody |
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| abx026744-80l | Abbexa | 80 µl | EUR 343.2 |
Mdm1 Nuclear Protein (MDM1) Antibody |
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| 20-abx327975 | Abbexa |
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Poly(A) Binding Protein, Nuclear 1 Protein |
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| 20-abx261243 | Abbexa |
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Rat Nuclear Receptor Coactivator 3 (NCOA3) Protein |
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| 20-abx168543 | Abbexa |
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Rat Embryo PrimaCell2: Normal Fetal Dermal Fibroblasts Growth Medium |
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| 9-25032 | CHI Scientific | 5 x 100 ml | Ask for price |
Rat Nuclear transition protein 2(TNP2) ELISA kit |
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| E02N0577-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rat Nuclear transition protein 2(TNP2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear transition protein 2(TNP2) ELISA kit |
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| E02N0577-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rat Nuclear transition protein 2(TNP2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear transition protein 2(TNP2) ELISA kit |
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| E02N0577-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rat Nuclear transition protein 2(TNP2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Embryo PrimaCell1: Normal Embryonic Chorion Cells Growth Medium |
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| 9-32030 | CHI Scientific | 5 x 100 ml | Ask for price |
Human Embryo PrimaCell1: Normal Embryonic Chorion Cells Growth Medium |
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| 9-46050 | CHI Scientific | 5 x 100 ml | Ask for price |
GTP-Binding Nuclear Protein RAN (RAN) Protein |
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| 20-abx262891 | Abbexa |
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Rat Embryo FibrOut1: Fibroblast Inhibitory System (for 500 ml medium) |
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| 7-15214 | CHI Scientific | 1 Kit | Ask for price |
Rat Embryo FibrOut3: Fibroblast Inhibitory System (for 500 ml medium) |
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| 7-15215 | CHI Scientific | 1 Kit | Ask for price |
Rat Embryo FibrOut4: Fibroblast Inhibitory System (for 500 ml medium) |
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| 7-15216 | CHI Scientific | 1 Kit | Ask for price |
Rat Nuclear migration protein nudC (NUDC) ELISA Kit |
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| abx391735-96tests | Abbexa | 96 tests | EUR 1093.2 |
Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat), PE |
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| 4-PAC791Ra01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with PE. |
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Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat), APC |
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| 4-PAC791Ra01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with APC. |
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Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat), Cy3 |
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| 4-PAC791Ra01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with Cy3. |
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Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat), HRP |
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| 4-PAC791Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with HRP. |
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Rat Cysteine/serine rich nuclear protein 2(CSRNP2) ELISA kit |
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| E02C2113-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rat Cysteine/serine rich nuclear protein 2(CSRNP2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Cysteine/serine rich nuclear protein 2(CSRNP2) ELISA kit |
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| E02C2113-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rat Cysteine/serine rich nuclear protein 2(CSRNP2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Cysteine/serine rich nuclear protein 2(CSRNP2) ELISA kit |
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| E02C2113-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rat Cysteine/serine rich nuclear protein 2(CSRNP2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Cysteine/serine rich nuclear protein 3(CSRNP3) ELISA kit |
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| E02C2114-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rat Cysteine/serine rich nuclear protein 3(CSRNP3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Cysteine/serine rich nuclear protein 3(CSRNP3) ELISA kit |
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| E02C2114-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rat Cysteine/serine rich nuclear protein 3(CSRNP3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Cysteine/serine rich nuclear protein 3(CSRNP3) ELISA kit |
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| E02C2114-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rat Cysteine/serine rich nuclear protein 3(CSRNP3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat), FITC |
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| 4-PAC791Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with FITC. |
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Human Thymocyte Nuclear Protein 1 (THYN1) Protein |
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| 20-abx650181 | Abbexa |
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Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b) Protein |
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| 20-abx653732 | Abbexa |
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Nuclear Protein Hcc-1 (HCC1) Antibody |
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| abx217271-100ug | Abbexa | 100 ug | EUR 526.8 |
Mouse Embryo PrimaCell4: Normal Trophoblast Cells |
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| 2-82033 | CHI Scientific | 1 Kit | Ask for price |
Human Embryo PrimaCell4: Normal Trophoblast Cells |
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| 2-96053 | CHI Scientific | 1 Kit | Ask for price |
Human Embryo OptiTDS1: Tissue Dissociation System |
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| 4-28050 | CHI Scientific | 1 Kit | Ask for price |
Human Embryo OptiTDS2: Tissue Dissociation System |
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| 4-28051 | CHI Scientific | 1 Kit | Ask for price |
Human Embryo OptiTDS3: Tissue Dissociation System |
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| 4-28052 | CHI Scientific | 1 Kit | Ask for price |
Human Embryo OptiTDS4: Tissue Dissociation System |
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| 4-28053 | CHI Scientific | 1 Kit | Ask for price |
Mouse Embryo OptiTDS1: Tissue Dissociation System |
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| 4-28144 | CHI Scientific | 1 Kit | Ask for price |
Mouse Embryo OptiTDS2: Tissue Dissociation System |
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| 4-28145 | CHI Scientific | 1 Kit | Ask for price |
Mouse Embryo OptiTDS3: Tissue Dissociation System |
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| 4-28146 | CHI Scientific | 1 Kit | Ask for price |
Mouse Embryo OptiTDS4: Tissue Dissociation System |
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| 4-28147 | CHI Scientific | 1 Kit | Ask for price |
Rat Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes Growth Medium |
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| 9-25033 | CHI Scientific | 5 x 100 ml | Ask for price |
Rat Embryo PrimaCell1: Normal Embryonic Chorion Cells Growth Supplements with Serum (for 500 ml medium) |
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| 9-26031 | CHI Scientific | 1 Set | Ask for price |
Human BTG3 Associated Nuclear Protein (BANP) Protein |
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| 20-abx652714 | Abbexa |
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Human Embryo Tissue Preparation Buffer 1: Normal Embryonic Chorion Cells |
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| 9-80050 | CHI Scientific | 1 x 100 ml | Ask for price |
Mouse Embryo Tissue Preparation Buffer 1: Normal Embryonic Chorion Cells |
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| 9-80144 | CHI Scientific | 1 x 100 ml | Ask for price |
Rat Nuclear Receptor Interacting Protein 1 ELISA kit |
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| E02N0045-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear Receptor Interacting Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear Receptor Interacting Protein 1 ELISA kit |
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| E02N0045-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear Receptor Interacting Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Nuclear Receptor Interacting Protein 1 ELISA kit |
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| E02N0045-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Nuclear Receptor Interacting Protein 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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ELISA kit for Rat Nuclear transition protein 2 (TNP2) |
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| KTE100054-48T | Abbkine | 48T | EUR 398.4 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear transition protein 2 (TNP2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Rat Nuclear transition protein 2 (TNP2) |
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| KTE100054-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear transition protein 2 (TNP2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Rat Nuclear transition protein 2 (TNP2) |
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| KTE100054-96T | Abbkine | 96T | EUR 646.8 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear transition protein 2 (TNP2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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Rat Nuclear Autoantigenic Sperm Protein (NASP) ELISA Kit |
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| 20-abx391725 | Abbexa |
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Rat Embryo Tissue Preparation Buffer 2: Normal Fetal Dermal Fibroblasts |
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| 9-80239 | CHI Scientific | 1 x 100 ml | Ask for price |
Melanoma Nuclear Protein 18 (MEL18) Antibody |
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| abx430561-200ul | Abbexa | 200 ul | EUR 460.8 |
RAN Antibody / Ras-related nuclear protein |
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| R32825 | NSJ Bioreagents | 100 ug | EUR 356.15 |
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Description: RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. |
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Mouse Nuclear protein 1, Nupr1 ELISA KIT |
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| ELI-36782m | Lifescience Market | 96 Tests | EUR 1038 |
Human Nuclear protein 1, NUPR1 ELISA KIT |
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| ELI-13386h | Lifescience Market | 96 Tests | EUR 988.8 |
Thymocyte Nuclear Protein 1 (THYN1) Antibody |
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| abx238682-100ug | Abbexa | 100 ug | EUR 610.8 |
Thymocyte Nuclear Protein 1 (THYN1) Antibody |
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| 20-abx211306 | Abbexa |
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Thymocyte Nuclear Protein 1 (THYN1) Antibody |
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| 20-abx116090 | Abbexa |
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Thymocyte Nuclear Protein 1 (THYN1) Antibody |
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| 20-abx131638 | Abbexa |
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Thymocyte Nuclear Protein 1 (THYN1) Antibody |
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| 20-abx131639 | Abbexa |
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Thymocyte Nuclear Protein 1 (THYN1) Antibody |
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| 20-abx339588 | Abbexa |
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Rat Nuclear Autoantigenic Sperm Protein, Histone Binding (NASP) Protein |
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| 20-abx650104 | Abbexa |
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Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat), Biotinylated |
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| 4-PAC791Ra01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with Biotin. |
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Human Mdm1 Nuclear Protein (MDM1) ELISA Kit |
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| abx381384-96tests | Abbexa | 96 tests | EUR 1093.2 |
Mouse Nuclear protein MDM1, Mdm1 ELISA KIT |
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| ELI-08463m | Lifescience Market | 96 Tests | EUR 1038 |
Human Nuclear protein MDM1, MDM1 ELISA KIT |
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| ELI-20467h | Lifescience Market | 96 Tests | EUR 988.8 |
Rat PEST proteolytic signal-containing nuclear protein (PCNP) ELISA Kit |
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| abx391793-96tests | Abbexa | 96 tests | EUR 1093.2 |
Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAC791Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with APC-Cy7. |
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Smad Nuclear Interacting Protein 1 Protein |
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| 20-abx261342 | Abbexa |
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Rat Embryo Tissue Preparation Buffer 3: Normal Fetal Epidermal Keratinocytes |
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| 9-80240 | CHI Scientific | 1 x 100 ml | Ask for price |
Flystuff (47-102) Grape Agar For Embryo Collection |
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| FLY1082 | Scientific Laboratory Supplies | PK25 | EUR 362.96 |
Rat Nuclear Mitotic AppaRatus Protein 1(NUMA1)ELISA Kit |
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| QY-E10566 | Qayee Biotechnology | 96T | EUR 433.2 |
BTG3 Associated Nuclear Protein (BANP) Antibody |
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| abx230799-100ug | Abbexa | 100 ug | EUR 610.8 |
BTG3 Associated Nuclear Protein (BANP) Antibody |
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| 20-abx175631 | Abbexa |
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BTG3 Associated Nuclear Protein (BANP) Antibody |
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| 20-abx171483 | Abbexa |
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BTG3 Associated Nuclear Protein (BANP) Antibody |
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| 20-abx321572 | Abbexa |
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BTG3 Associated Nuclear Protein (BANP) Antibody |
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| 20-abx111276 | Abbexa |
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BTG3 Associated Nuclear Protein (BANP) Antibody |
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| 20-abx006024 | Abbexa |
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BTG3 Associated Nuclear Protein (BANP) Antibody |
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| 20-abx003640 | Abbexa |
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Nuclear Transport Factor 2 Protein |
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| 20-abx260609 | Abbexa |
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Sp110 Nuclear Body Protein Antibody |
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| 20-abx115748 | Abbexa |
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Nuclear Matrix Protein p84 antibody |
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| 70R-13662 | Fitzgerald | 100 ul | EUR 548.4 |
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Description: Affinity purified Rabbit polyclonal Nuclear Matrix Protein p84 antibody |
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Nuclear Matrix Protein p84 Antibody |
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| 49738-100ul | SAB | 100ul | EUR 399.6 |
Nuclear Matrix Protein p84 Antibody |
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| 49738-50ul | SAB | 50ul | EUR 286.8 |
Human GTP-binding nuclear protein Ran (RAN) |
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| 1-CSB-EP019307HU | Cusabio |
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Description: Recombinant Human GTP-binding nuclear protein Ran(RAN) expressed in E.coli |
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Chicken Nuclear protein MDM1, MDM1 ELISA KIT |
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| ELI-22770c | Lifescience Market | 96 Tests | EUR 1113.6 |
Anti-P34 cdc-2 Nuclear Protein antibody |
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| STJ16100759 | St John's Laboratory | 100 µg | EUR 487.2 |
Cytoplasmic and Nuclear Protein Extraction Kit |
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| AR0106 | BosterBio | 1kit | EUR 121.2 |
Recombinant Human Thymocyte Nuclear Protein 1 |
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| 7-06664 | CHI Scientific | 5µg | Ask for price |
Recombinant Human Thymocyte Nuclear Protein 1 |
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| 7-06665 | CHI Scientific | 20µg | Ask for price |
Recombinant Human Thymocyte Nuclear Protein 1 |
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| 7-06666 | CHI Scientific | 1mg | Ask for price |
Recombinant Thymocyte Nuclear Protein 1 (THYN1) |
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| 4-RPC791Hu01 | Cloud-Clone |
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Description: Recombinant Human Thymocyte Nuclear Protein 1 expressed in: E.coli |
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Recombinant Thymocyte Nuclear Protein 1 (THYN1) |
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| 4-RPC791Ra01 | Cloud-Clone |
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Description: Recombinant Rat Thymocyte Nuclear Protein 1 expressed in: E.coli |
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Rat Smad nuclear-interacting protein 1 (SNIP1) ELISA Kit |
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| abx391974-96tests | Abbexa | 96 tests | EUR 1093.2 |
Recombinant Ebola-Z Nuclear Protein (aa 630-739) |
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| VAng-Lsx0146-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Ebola-Z nuclear protein(aa 630-739), Recombinant from E. coli. |
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Recombinant Ebola-S Nuclear Protein (aa 629-728) |
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| VAng-Lsx0147-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Ebola-S nuclear protein (aa 629-728), Recombinant from E. coli. |
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ELISA kit for Rat Nuclear autoantigenic sperm protein (NASP) |
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| KTE100520-48T | Abbkine | 48T | EUR 398.4 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear autoantigenic sperm protein (NASP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Rat Nuclear autoantigenic sperm protein (NASP) |
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| KTE100520-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear autoantigenic sperm protein (NASP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Rat Nuclear autoantigenic sperm protein (NASP) |
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| KTE100520-96T | Abbkine | 96T | EUR 646.8 |
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Description: Quantitative sandwich ELISA for measuring Rat Nuclear autoantigenic sperm protein (NASP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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Human Nuclear Matrix Protein 4 (NMP4) Protein |
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| 20-abx165940 | Abbexa |
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Rat Nuclear Receptor Interacting Protein 1 (NRIP1) ELISA Kit |
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| abx353802-96tests | Abbexa | 96 tests | EUR 801.6 |
GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx242019 | Abbexa |
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GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx242241 | Abbexa |
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GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx214680 | Abbexa |
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GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx214681 | Abbexa |
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GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx119043 | Abbexa |
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GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx321684 | Abbexa |
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GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| abx032938-400ul | Abbexa | 400 ul | EUR 627.6 |
GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| abx032938-80l | Abbexa | 80 µl | EUR 343.2 |
GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx127029 | Abbexa |
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GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx328361 | Abbexa |
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GTP-Binding Nuclear Protein RAN (RAN) Antibody |
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| 20-abx000929 | Abbexa |
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Rat Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) Protein |
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| 20-abx166221 | Abbexa |
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Xpert Nuclear Protein Extraction Kit, Nucleic acid free |
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| N2200-050 | GenDepot | 50 ext | Ask for price |
Xpert Nuclear Protein Extraction Kit, Nucleic acid free |
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| N2200-100 | GenDepot | 100 ext | Ask for price |
Mouse Embryo PrimaCell2: Normal Fetal Dermal Fibroblasts |
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| 2-82031 | CHI Scientific | 1 Kit | Ask for price |
Human Embryo PrimaCell2: Normal Fetal Dermal Fibroblasts |
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| 2-96051 | CHI Scientific | 1 Kit | Ask for price |
THYN1 Thymocyte Nuclear Protein 1 Human Recombinant Protein |
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| PROTQ9P016 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: THYN1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 245 amino acids (1-225a.a.) and having a molecular mass of 27.8kDa.;THYN1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
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ELISA kit for Mouse Nuclear protein MDM1 (MDM1) |
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| KTE71082-48T | Abbkine | 48T | EUR 398.4 |
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Description: Quantitative sandwich ELISA for measuring Mouse Nuclear protein MDM1 (MDM1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Mouse Nuclear protein MDM1 (MDM1) |
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| KTE71082-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
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Description: Quantitative sandwich ELISA for measuring Mouse Nuclear protein MDM1 (MDM1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Mouse Nuclear protein MDM1 (MDM1) |
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| KTE71082-96T | Abbkine | 96T | EUR 646.8 |
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Description: Quantitative sandwich ELISA for measuring Mouse Nuclear protein MDM1 (MDM1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Human Nuclear protein MDM1 (MDM1) |
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| KTE61677-48T | Abbkine | 48T | EUR 398.4 |
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Description: Quantitative sandwich ELISA for measuring Human Nuclear protein MDM1 (MDM1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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In an effort to make clear the compositions of NCBP1-, 2- and 3-related macromolecular assemblies, now we have utilized an affinity capture-based interactome display the place the experimental design and information processing have been modified to quantitatively establish interactome variations between targets beneath a variety of experimental situations. This examine generated a complete view of NCBP-protein interactions within the ribonucleoprotein context and demonstrates the potential of our method to profit the interpretation of complicated organic pathways.